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Chemical & Biological Processing

Ellen Panisko

Ellen Panisko

Pacific Northwest National Laboratory
PO Box 999
Richland, WA 99352


Dr. Panisko currently uses proteomic and molecular biology methods to study fungal morphology and hyperproductivity. She is experienced with stable isotope labeling strategies and affinity purification of peptides for mass spectral analysis. One of her current projects focuses on the contribution of technical and biological variability to relative protein concentration determined by high throughput proteome analyses. Her graduate and postdoctoral research experience has included expression of heterologous proteins in plant tissues and investigating the kinetics of a tricarboxylic acid cycle enzyme in Saccharaomyces cerevisiae. In 2002, she became a member of the Fungal Biotechnology team.

Education and Credentials

  • Ph.D., Biochemistry, University of Texas Health Science Center at San Antonio, 2000
  • B.S., Biochemistry, cum laude, Washington State University, 1995

PNNL Publications


  • Collett J.R., J.M. Billing, P.A. Meyer, A.J. Schmidt, A.B. Remington, E. Hawley, and B.A. Hofstad, et al. 2019. "Renewable diesel via hydrothermal liquefaction of oleaginous yeast and residual lignin from bioconversion of corn stover." Applied Energy 233. PNNL-SA-133222. doi:10.1016/j.apenergy.2018.09.115


  • Albrecht K.O., R.A. Dagle, D.T. Howe, J.A. Lopez-Ruiz, S.D. Davidson, B. Maddi, and A.R. Cooper, et al. 2018. Final Report for the Project Characterization and Valorization of Aqueous Phases Derived from Liquefaction and Upgrading of Bio-Oils. PNNL-27848. Richland, WA: Pacific Northwest National Laboratory.
  • Presley G.N., E.A. Panisko, S.O. Purvine, and J.S. Schilling. 2018. "Coupling Secretomics with Enzyme Activities To Compare the Temporal Processes of Wood Metabolism among White and Brown Rot Fungi." Applied and Environmental Microbiology 84, no. 16:Article No. e00159-18. PNNL-SA-135357. doi:10.1128/AEM.00159-18
  • Weber R.S., J.E. Holladay, C. Jenks, E.A. Panisko, L.J. Snowden-Swan, M. Ramirez-Corresdores, and B. Baynes, et al. 2018. "Modularized production of fuels and other value-added products from distributed, wasted, or stranded feedstocks." Wiley Interdisciplinary Reviews: Energy and Environment 7, no. 6:e308. PNNL-SA-131136. doi:10.1002/wene.308


  • Maddi B., E.A. Panisko, T.W. Wietsma, T.L. Lemmon, M.S. Swita, K.O. Albrecht, and D.T. Howe. 2017. "Quantitative Characterization of Aqueous Byproducts from Hydrothermal Liquefaction of Municipal Wastes, Food Industry Wastes, and Biomass Grown on Waste." ACS Sustainable Chemistry & Engineering 5, no. 3:2205-2214. PNNL-SA-121413. doi:10.1021/acssuschemeng.6b02367
  • Unkefer C.J., R.T. Sayre, J.K. Magnuson, D.B. Anderson, I. Baxter, I.K. Blaby, and J.K. Brown, et al. 2017. "Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts." Algal Research 22. PNNL-SA-125672. doi:10.1016/j.algal.2016.06.002


  • Maddi B., E.A. Panisko, K.O. Albrecht, and D.T. Howe. 2016. "Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry." Journal of Visualized Experiments 109. PNNL-SA-109916. doi:10.3791/53634
  • Maddi B., E.A. Panisko, T.W. Wietsma, T.L. Lemmon, M.S. Swita, K.O. Albrecht, and D.T. Howe. 2016. "Quantitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae." Biomass & Bioenergy 93. PNNL-SA-113775. doi:10.1016/j.biombioe.2016.07.010


  • Panisko E.A., B. Maddi, K.O. Albrecht, and D.T. Howe. 2015. "Characterization of the aqueous fraction from hydrothermal liquefaction of algae using 2D gas chromatography with time-of-flight mass spectrometry." In 2015 AIChE Annual Meeting, November 8-13, 2015, Salt Lake City, UT. New York, New York:American Institute of Chemical Engineers. PNNL-SA-110289.
  • Panisko E.A., T.W. Wietsma, T.L. Lemmon, K.O. Albrecht, and D.T. Howe. 2015. "Characterization of the Aqueous Fractions from Hydrotreatment and Hydrothermal Liquefaction of Lignocellulosic Feedstocks." Biomass & Bioenergy 74. PNNL-SA-105768. doi:10.1016/j.biombioe.2015.01.011


  • Wiedner S.D., C. Ansong, B.M. Webb-Robertson, L.M. Pederson, S. Fortuin, B.A. Hofstad, and A.K. Shukla, et al. 2013. "Disparate Proteome Responses of Pathogenic and Non-pathogenic Aspergilli to Human Serum Measured by Activity-Based Protein Profiling (ABPP)." Molecular & Cellular Proteomics. MCP 12, no. 7:1791-1805. PNNL-SA-91969. doi:10.1074/mcp.M112.026534


  • Wiedner S.D., K.E. Burnum, L.M. Pederson, L.N. Anderson, S. Fortuin, L.M. Chauvigne-Hines, and A.K. Shukla, et al. 2012. "Multiplexed Activity-based Protein Profiling of the Human Pathogen Aspergillus fumigatus Reveals Large Functional Changes upon Exposure to Human Serum." Journal of Biological Chemistry 287, no. 40:33447-33459. PNNL-SA-87258. doi:10.1074/jbc.M112.394106


  • Baker S.E., and E.A. Panisko. 2011. "Proteome Studies of Filamentous Fungi." In Fungal Genomics: Methods and Protocols. Methods in Molecular Biology, edited by JR Xu and BHH Bluhm. 133-139. New York, New York:Springer. PNNL-SA-78038.
  • de Vries R.P., I. Benoit, G. Doehlemann, T. Kobayashi, J.K. Magnuson, E.A. Panisko, and S.E. Baker, et al. 2011. "Post-genomic approaches to understanding interactions between fungi and their environment." IMA Fungus 2, no. 1:81-86. PNNL-SA-80280. doi:10.5598/imafungus.2011.02.01.12
  • Ryu J.S., S. Shary, C.J. Houtman, E.A. Panisko, P. Korripally, F.J. St John, and C. Crooks, et al. 2011. "Proteomic and Functional Analysis of the Cellulase System Expressed by Postia placenta during Brown Rot of Solid Wood." Applied and Environmental Microbiology 77, no. 22:7933-7941. PNNL-SA-84670. doi:10.1128/AEM.05496-11


  • Baker S.E., K.S. Bruno, M.G. Butcher, J.R. Collett, D.E. Culley, Z. Dai, and J.K. Magnuson, et al. 2009. PNNL Fungal Biotechnology Core DOE-OBP Project. PNNL-18820. Richland, WA: Pacific Northwest National Laboratory. PNNL Fungal Biotechnology Core DOE-OBP Project
  • Tsang A., G.D. Butler, G.D. Butler, J. Powlowski, E.A. Panisko, and S.E. Baker. 2009. "Analytical and computational approaches to define the Aspergillus niger secretome." Fungal Genetics and Biology : FG & B 46, no. 1, Suppl. 1:S153-S160. PNNL-SA-65695. doi:10.1016/j.fgb.2008.07.014


  • Daly D.S., K.K. Anderson, E.A. Panisko, S.O. Purvine, R. Fang, M.E. Monroe, and S.E. Baker. 2008. "A mixed-effects Statistical Model for Comparative LC-MS Proteomics Studies." Journal of Proteome Research 7, no. 3:1209-1217. PNNL-SA-57824. doi:10.1021/pr070441i
  • Shary S., A.N. Kapich, A.N. Kapich, E.A. Panisko, J.K. Magnuson, D. Cullen, and K. Hammel, et al. 2008. "Differential Expression in Phanerochaete chrysosporium of Membrane-Associated Proteins Relevant to Lignin Degradation." Applied and Environmental Microbiology 74, no. 23:7252-7257. PNNL-SA-63951. doi:10.1128/AEM.01997-08
  • Valentine N.B., M.G. Butcher, Y. Su, K.H. Jarman, M.M. Matzke, B.M. Webb-Robertson, and E.A. Panisko, et al. 2008. "Evaluation of Sampling Tools for Environmental Sampling of Bacterial Endospores from Porous and Non-porous Surfaces." Journal of Applied Microbiology 105, no. 4:1107-1113. PNNL-SA-60777.


  • Baker S.E., C.F. Wend, A.D. Martinez, J.K. Magnuson, E.A. Panisko, Z. Dai, and K.S. Bruno, et al. 2007. "Genome and Proteome Analysis of Industrial Fungi." In Exploitation of Fungi, edited by GD Robson, P van West and G Gadd. 3-9. New York, New York:Cambridge University Press. PNNL-SA-51617. doi:10.1017/CBO9780511902451.002


  • Goshe M., T.D. Veenstra, E.A. Panisko, T.P. Conrads, N.H. Angell, and R.D. Smith. 2002. "Phosphoprotein Isotope-coded Affinity Tags: Application to the Enrichment and Identification of Low-Abundance Phosphoproteins." Analytical Chemistry 74, no. 3:607-616. PNWD-SA-5412.
  • Panisko E.A., T.P. Conrads, M.B. Goshe, and T.D. Veenstra. 2002. "The Postgenomic Age: Characterization of Proteomes." Experimental Hematology 30, no. 2:97-107. PNNL-SA-34715.


  • Goshe M.B., T.P. Conrads, E.A. Panisko, N.H. Angell, T.D. Veenstra, and R.D. Smith. 2001. "Phosphoprotein Isotope-coded Affinity Tag (PhIAT) Approach for Isolating and Quantitating Phosphopeptides in Proteome-wide Analyses." Analytical Chemistry 73, no. 11:2578-2586. PNNL-SA-34061.

Chemical & Biological Processing

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