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Chemical and Biological Process Development

Ellen Panisko

Ellen Panisko

Pacific Northwest National Laboratory
PO Box 999
Richland, WA 99352

Biography

Dr. Panisko currently uses proteomic and molecular biology methods to study fungal morphology and hyperproductivity. She is experienced with stable isotope labeling strategies and affinity purification of peptides for mass spectral analysis. One of her current projects focuses on the contribution of technical and biological variability to relative protein concentration determined by high throughput proteome analyses. Her graduate and postdoctoral research experience has included expression of heterologous proteins in plant tissues and investigating the kinetics of a tricarboxylic acid cycle enzyme in Saccharaomyces cerevisiae. In 2002, she became a member of the Fungal Biotechnology team.

Education and Credentials

  • Ph.D., Biochemistry, University of Texas Health Science Center at San Antonio, 2000
  • B.S., Biochemistry, cum laude, Washington State University, 1995

PNNL Publications

2017

  • Maddi B, EA Panisko, TW Wietsma, TL Lemmon, MS Swita, KO Albrecht, and DT Howe. 2017. "Quantitative Characterization of Aqueous Byproducts from Hydrothermal Liquefaction of Municipal Wastes, Food Industry Wastes, and Biomass Grown on Waste." ACS Sustainable Chemistry & Engineering 5(3):2205-2214.  doi:10.1021/acssuschemeng.6b02367
  • Unkefer CJ, RT Sayre, JK Magnuson, DB Anderson, I Baxter, IK Blaby, JK Brown, M Carleton, RA Cattolico, T Dale, TP Devarenne, CM Downes, SK Dutcher, DT Fox, UW Goodenough, J Jaworski, JE Holladay, DM Kramer, AT Koppisch, MS Lipton, BL Marrone, MM Mccormick, I Molnar, JB Mott, KL Ogden, EA Panisko, M Pellegrini, JE Polle, JW Richardson, M Sabarskyo, SR Starkenburg, GD Stormo, M Teshima, S Twary, PJ Unkefer, J Yuan, and JéA Olivares. 2017. "Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts." Algal Research 22:187-215.  doi:10.1016/j.algal.2016.06.002

2016

  • Maddi B, EA Panisko, KO Albrecht, and DT Howe. 2016. "Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry." Journal of Visualized Experiments 109:e53634.  doi:10.3791/53634
  • Maddi B, EA Panisko, TW Wietsma, TL Lemmon, MS Swita, KO Albrecht, and DT Howe. 2016. "Quantitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae." Biomass & Bioenergy 93:122-130.  doi:10.1016/j.biombioe.2016.07.010

2015

  • Panisko EA, TW Wietsma, TL Lemmon, KO Albrecht, and DT Howe. 2015. "Characterization of the Aqueous Fractions from Hydrotreatment and Hydrothermal Liquefaction of Lignocellulosic Feedstocks." Biomass & Bioenergy 74:162-171.  doi:10.1016/j.biombioe.2015.01.011

2013

  • Wiedner SD, C Ansong, BJM Webb-Robertson, LM Pederson, S Fortuin, BA Hofstad, AK Shukla, EA Panisko, RD Smith, and AT Wright. 2013. "Disparate Proteome Responses of Pathogenic and Non-pathogenic Aspergilli to Human Serum Measured by Activity-Based Protein Profiling (ABPP)." Molecular & Cellular Proteomics. MCP 12(7):1791-1805.  doi:10.1074/mcp.M112.026534

2012

  • Wiedner SD, KE Burnum, LM Pederson, LN Anderson, S Fortuin, LM Chauvigne-Hines, AK Shukla, C Ansong, EA Panisko, RD Smith, and AT Wright. 2012. "Multiplexed Activity-based Protein Profiling of the Human Pathogen Aspergillus fumigatus Reveals Large Functional Changes upon Exposure to Human Serum." Journal of Biological Chemistry 287(40):33447-33459.  doi:10.1074/jbc.M112.394106
  • Wiedner SD, KE Burnum, LM Pederson, LN Anderson, S Fortuin, LM Chauvigne-Hines, AK Shukla, C Ansong, EA Panisko, RD Smith, and AT Wright. 2012. "Multiplexed Activity-Based Protein Profiling of the Human Pathogen Aspergillus fumigatus Reveals Large Functional Changes Upon Exposure to Human Serum." Journal of Biological Chemistry 287(40):33447-33459.  doi:10.1074/jbc.M112.394106

2011

  • Baker SE, and EA Panisko. 2011. "Proteome Studies of Filamentous Fungi." Chapter 9 in Fungal Genomics: Methods and Protocols. Methods in Molecular Biology, vol. 722, ed. JR Xu and BHH Bluhm, pp. 133-139.  Springer, New York, NY. 
  • de Vries RP, I Benoit, G Doehlemann, T Kobayashi, JK Magnuson, EA Panisko, SE Baker, and MH Lebrun. 2011. "Post-genomic approaches to understanding interactions between fungi and their environment." IMA Fungus 2(1):81-86.  doi:10.5598/imafungus.2011.02.01.12
  • Ryu JS, S Shary, CJ Houtman, EA Panisko, P Korripally, FJ St John, C Crooks, M Siika-aho, JK Magnuson, and K Hammel. 2011. "Proteomic and Functional Analysis of the Cellulase System Expressed by Postia placenta during Brown Rot of Solid Wood." Applied and Environmental Microbiology 77(22):7933-7941.  doi:10.1128/AEM.05496-11

2009

  • Baker SE, KS Bruno, MG Butcher, JR Collett, DE Culley, Z Dai, JK Magnuson, and EA Panisko. 2009. PNNL Fungal Biotechnology Core DOE-OBP ProjectPNNL-18820, Pacific Northwest National Laboratory, Richland, WA. 
  • Tsang A, GD Butler, J Powlowski, EA Panisko, and SE Baker. 2009. "Analytical and computational approaches to define the Aspergillus niger secretome." Fungal Genetics and Biology : FG & B 46(1, Suppl. 1):S153-S160.  doi:10.1016/j.fgb.2008.07.014

2008

  • Daly DS, KK Anderson, EA Panisko, SO Purvine, R Fang, ME Monroe, and SE Baker. 2008. "A mixed-effects Statistical Model for Comparative LC-MS Proteomics Studies." Journal of Proteome Research 7(3):1209-1217.  doi:10.1021/pr070441i
  • Shary S, AN Kapich, EA Panisko, JK Magnuson, D Cullen, and K Hammel. 2008. "Differential Expression in Phanerochaete chrysosporium of Membrane-Associated Proteins Relevant to Lignin Degradation." Applied and Environmental Microbiology 74(23):7252-7257.  doi:10.1128/AEM.01997-08
  • Valentine NB, MG Butcher, YF Su, KH Jarman, MM Matzke, BJM Webb-Robertson, EA Panisko, BAB Seiders, and KL Wahl. 2008. "Evaluation of Sampling Tools for Environmental Sampling of Bacterial Endospores from Porous and Non-porous Surfaces." Journal of Applied Microbiology 105 (4):1107-1113. 

2007

  • Baker SE, CF Wend, AD Martinez, JK Magnuson, EA Panisko, Z Dai, KS Bruno, KK Anderson, ME Monroe, DS Daly, and LL Lasure. 2007. "Genome and Proteome Analysis of Industrial Fungi." Chapter 1 in Exploitation of Fungi, ed. GD Robson, P van West and G Gadd, pp. 3-9.  Cambridge University Press, New York, NY.  doi:10.1017/CBO9780511902451.002

2002

  • Panisko EA, TP Conrads, MB Goshe, and TD Veenstra. 2002. "The Postgenomic Age: Characterization of Proteomes." Experimental Hematology 30(2):97-107. 
  • Goshe M, TD Veenstra, EA Panisko, TP Conrads, NH Angell, and RD Smith. 2002. "Phosphoprotein Isotope-coded Affinity Tags: Application to the Enrichment and Identification of Low-Abundance Phosphoproteins." Analytical Chemistry 74(3):607-616. 

2001

  • Goshe MB, TP Conrads, EA Panisko, NH Angell, TD Veenstra, and RD Smith. 2001. "Phosphoprotein Isotope-coded Affinity Tag (PhIAT) Approach for Isolating and Quantitating Phosphopeptides in Proteome-wide Analyses." Analytical Chemistry 73(11):2578-2586. 

Chemical and Biological Process Development

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